Composite

Part:BBa_K1615013

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-27)

MorA fused to CBDcenA+Linker in RFC25 driven by LacI promoter

Morphine-6-dehydrogenase fused to the N terminal of CBDcenA + C terminal linker (BBa_K1615003) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the morphine-6-dehydrogenase CBD fusion to express when induced with IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None